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For the thio-amide substrates, the Cd-enzyme was the ideal enzyme and the Zn-enzyme was poorer. The catalytic constants for your Zn- and Cd-substituted types of NagA assist the summary the carbonyl team with the substrate is polarized by a direct interaction with The only divalent cation certain to the Mβ-placement while in the Lively web site.

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enzyme can bind but one divalent cation in the Energetic website. For a binuclear steel center, the activation of your amide bond plus the activation from the solvent drinking water might be dispersed separately concerning the two metallic ions. Even so, during the mononuclear metallic center, both of those functions should be borne by one divalent cation. These final results spotlight the numerous diversity to the evolution of function within the amidohydrolase superfamily.

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This observation suggests that There was a divergence in the evolution of NagA and indicates that there are fundamental distinctions while in the mechanistic information for substrate activation and hydrolysis. NagA from E. coli

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